Introduction Various bioinformatic tools enable analysis of nucleic acid sequences and so faciliate identification of specific signals, motifs and sequences within them, e.g.: - promoters
- terminators
- RBSs
- splicing sites
- START a STOP codons
- polyA signals
- restriction sites
- coding sequences
Task no. 1 Identify cleavage sites for restriction endonucleases EcoRI and EcoRV in EMBL entry AJ238043 using tool Restrict. Then try to find out: - number and position of restriction sites for individual restriction endonucleases
- number and length of frangments at simultaneous cleavage with both endonucleases
- what kind of ends are produced after cleavage with these endonucleases
- what are the source organisms for these endonucleases
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