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4. Analysis of biological sequences

Introduction

Tools for statistical analysis of DNA and protein sequences convey information e.g. about:
  • nucleotide and aminoacid content in sequence
  • G+C content
  • molecular weight, hydrophobicity or charge of proteins
  • DNA melting temperature
  • relative codon and aminoacid usage

Task no. 1

Using tool Cusp make up codon usage table for genes of ribosomal protein L1 from Escherichia coli and Streptomyces aureofaciens. Then try to compare:
  1. realtive codon usage for aminoacid valine for these two microorganisms.
  2. What conclusion would you deduce from codon preference for valine according to systematic ranking of thses two microorganisms?
 
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Last update: 08.12.2022 Authors: Matej Stano and Lubos Klucar, Institute of Molecular Biology, SAS Bratislava
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